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nedd1 am  (Bethyl)
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Bethyl nedd1 am
Fig. 1. GFP-centrin-1 as a marker of IR- induced centrosome amplification. (A) Human U2OS cells expressing GFP-centrin-1 (green) and H2B-RFP (red) were fixed in methanol and stained with the following antibodies to centrosomal or centrosome-associated proteins, Aurora A, γ-tubulin, CEP170, Ninein and <t>Nedd1</t> (blue, Alexa 350). Scale bar, 10 mm. U2OS cells as described in (A), before (B) and after (C) treatment with 10 Gy IR.
Nedd1 Am, supplied by Bethyl, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Fig. 1. GFP-centrin-1 as a marker of IR- induced centrosome amplification. (A) Human U2OS cells expressing GFP-centrin-1 (green) and H2B-RFP (red) were fixed in methanol and stained with the following antibodies to centrosomal or centrosome-associated proteins, Aurora A, γ-tubulin, CEP170, Ninein and Nedd1 (blue, Alexa 350). Scale bar, 10 mm. U2OS cells as described in (A), before (B) and after (C) treatment with 10 Gy IR.

Journal: Cell cycle (Georgetown, Tex.)

Article Title: Involvement of centrosome amplification in radiation-induced mitotic catastrophe.

doi: 10.4161/cc.6.3.3834

Figure Lengend Snippet: Fig. 1. GFP-centrin-1 as a marker of IR- induced centrosome amplification. (A) Human U2OS cells expressing GFP-centrin-1 (green) and H2B-RFP (red) were fixed in methanol and stained with the following antibodies to centrosomal or centrosome-associated proteins, Aurora A, γ-tubulin, CEP170, Ninein and Nedd1 (blue, Alexa 350). Scale bar, 10 mm. U2OS cells as described in (A), before (B) and after (C) treatment with 10 Gy IR.

Article Snippet: Cells were fixed in either 4% PFA or methanol as described in ref. 22 and stained using the following primary antibodies: γ-tubulin (T3559, Sigma), ninein (AM), CEP170 (GG), Nedd1 (AM), Aurora A (35C1, Abcam), a-tubulin (B512, Sigma), TPX-2 (BL1679, Bethyl Labs), BubR1 (8G1, AbCam) and Mad2 (Abcam).

Techniques: Marker, Amplification, Expressing, Staining